Kylt® 2x qPCR-Mix is a ready-to-use, 2x concentrated Enzyme Reagent, that is to be combined with specific primer- and probe-setups for detection of DNA (e.g. viral DNA or bacterial DNA) by Real-Time Polymerase Chain Reaction (Real-Time PCR or qPCR).
The qualitative analysis of DNA is based on the amplification of target sequences by the primer pair(s) in the PCR. Amplified target gene fragments are detected via fluorescently labelled probes during the PCR reaction in Real-Time (Real-Time PCR).
The Kylt® 2x qPCR-Mix contains all essential components, such as DNA Polymerase, nucleotides and a suitable buffer system.
The components are provided in a proprietary buffer system, that enabled the optimal performance of the amplification as well as highest specificity and sensitivity of the target specific detection in Real-Time.