Kylt® 2x RT-qPCR-Mix is a ready-to-use, 2x concentrated Enzyme Reagent, that is to be combined with specific primer and probe-setups for the detection of RNA (e.g. viral RNA) by Real-Time Polymerase Chain Reaction (Real-Time PCR or
qPCR) following cDNA synthesis (performed by Reverse Transcription (RT)).
The subsequent qualitative analysis of cDNA is based on the amplifi cation of target sequences by the primer pair(s) in the PCR. Amplified target gene fragments are detected via fluorescently labelled probes during the PCR reaction in Real-Time.
The Kylt® 2x RT-qPCR-Mix contains all essential components, such as Reverse Transcriptase, DNA Polymerase, nucleotides and a suitable reaction buffer. The components are provided in a proprietary system, that enables the optimal performance of the Reverse Transcriptase and amplifi cation as well as highest specifi city and sensitivity of the target specific detection in Real-Time.