Universal Temperature Profiles for qPCR and RT-qPCR Assays
Two universal temperature profiles for all Kylt® Real-Time Detection products*
Profile I for Kylt® RT-qPCR e.g. Kylt® Influenza A and for Kylt® qPCR e.g. Kylt® MGS Triplex, if you would like to combine RT-qPCR and qPCR runs.
The (current) Profile II can still be used with Kylt® qPCR Detection Kits if no Reverse Transcription step is needed.
* For details of temperature profiles, please refer to the Direction For Use. There are two exceptions: Kylt® RT-qPCR for detection of different IB-Variants and Kylt® IBDV Serotype I run in a slightly different temperature profile and thus can not be combined with further Kylt® (RT-) qPCRs.
Universal Reaction Setup 16+4
16 μl Master-Mix + 4 μl DNA or RNA
To make the reaction setup as easy as possible we have adjusted the volumes of Master-Mix and final RNA/DNA preparations for the RT-qPCR and qPCR assays. Both assay types are now set up with 16 μl of Master-Mix plus 4 μl of DNA or RNA, respectively.
Kylt® DNA Extraction-Mix II
Kylt® DNA Extraction-Mix II will be removed from (Real-Time) PCR Detection Kits
The Mix is available separately (Kylt® DNA Extraction-Mix II, Art. No. 31398 for 100 reactions).
The Kylt® (Real-Time) PCR Detection products will replace our Kylt® DNA Extraction and (Real-Time) PCR Detection; the article numbers will stay the same, so you can use the order numbers as you are accustomed to.
As an you can use the Kylt® RNA / DNA Purification Kit (Art. No. 31314/31315 for 5x50/50 alternative reactions). This spin column-based kit allows simultaneous purification of RNA and DNA from a broad range of veterinary samples, like swabs, feces or blood.