SE DIVA 2 Image 1
SE DIVA 2
Salmonella Enteritidis field strain, Salmonella Enteritidis vaccine strain Sm24/Rif12/Ssq and CAL 10 Sm+/Rif+/Ssq-
Kylt SE DIVA 2 is intended for the differentiation of the following Salmonella Enteritidis (SE) live vaccine strains (SEV2) from field strains (SEf):
  • Name of the commercially available vaccine: AviPro SALMONELLA VAC E (and AviPro SALMONELLA DUO - please consider the note in the manual)
  • Name of the strain: Sm24/Rif12/Ssq
  • Name of the commercially available vaccine: Primun Salmonella E
  • Name of the strain: CAL 10 Sm+/Rif+/Ssq-

Kylt SE DIVA 2 is intended to be used for Salmonella spp. -positive material from cultural processes according to DIN EN ISO 6579 or equivalent cultivation methods.The kit contains all reagents and controls for the detection.

General

The kit is intended for the differentiation of the following Salmonella Enteritidis (SE) live vaccine strains (SEV2) from field strains (SEf):
  • Name of the commercially available vaccine: AviPro SALMONELLA VAC E (and AviPro SALMONELLA DUO - please consider the note below)
  • Name of the strain: Sm24/Rif12/Ssq
  • Name of the manufacturer: Elanco (Lohmann Animal Health GmbH)
  • Name of the commercially available vaccine: Primun Salmonella E
  • Name of the strain: CAL 10 Sm+/Rif+/Ssq-
  • Name of the manufacturer: CALIER
Article No.Article typeSize / ContentUnit
31161Kit100Reactions
31162Kit25Reactions
Article name: Kylt® SE DIVA 2 LD 100, Kylt® SE DIVA 2 LD 25
Name of pathogen: Salmonella Enteritidis field strain, Salmonella Enteritidis vaccine strain Sm24/Rif12/Ssq and CAL 10 Sm+/Rif+/Ssq-
Affected organs: Intestinal system
Organism: Bacteria
Target molecule: DNA
Technology: Real-Time PCR
Detection type: Serovar-specific detection and DIVA
Host-group: Poultry (Chicken, Turkey)
Sample prerequisite: Salmonella spp. 2.0
Target FAM channel (520 nm): Salmonella Enteritidis
Target Cy5 channel (670 nm): SE Sm24/Rif12/Ssq and CAL 10 Sm+/Rif+/Ssq-
Target TXR channel (620 nm): SE field strain
Detected vaccines: - Strain: Sm24/Rif12/Ssq, Vaccine: AviPro SALMONELLA VAC E and AviPro SALMONELLA DUO, Manufacturer: Elanco (Lohmann Animal Health GmbH), - Strain: CAL 10 Sm+/Rif+/Ssq-, Vaccine: Primun Salmonella E, Manufacturer: CALIER
Kylt storage temperature: <= -18°C
Sample type:
  • pre-enrichment samples positively tested for Salmonella spp. (Ct < 25), Salmonella spp. positive colony material from cultural processes (e.g. DIN EN ISO 6579), In case of samples with a positive result Ct > 25 in Salmonella spp. screening either a second enrichment step should be performed or the analysis should be repeated using colony material from microbiological culture
RNA/DNA Purification Purifier 96 Purifier 48 Salmonella Purification HTP RNA/DNA Purification HTP Salmonella spp. 2.0